LightWave™ - ECL HRP
Western blotting substrate
LightWave™ is our product line of ECL HRP substrates for Western blotting.
Our double enhancer proprietary technology allows for modulation of signal intensity and signal duration.
Each LightWave™ substrate is at the top of its respective market segment regarding performance/price ratio.
Properties
All LightWave substrates are:
- Stable for 1 year at RT
- Low picogram detection
- Ideal for routinary analysis
- Working solution stable for at least three days
- The best entry level ECL substrate on the market
- Signal duration 5 hours
LightWave™ has:
Signal intensity: medium
Signal duration: medium
Protein abundance: high
Storage/expiry: one year at room temperature (max. 25°C)
Characteristics
- Sample
- two-fold dilution series of Hela whole cell lysate (abcam®) from 5 μg to 0.078 μg of total protein
- Primary antibody
- rabbit-anti Human HDAC-1 (abcam®) 1:2000
- Secondary antibody
- goat anti-rabbit IgG HRP (2mg/mL) (abcam®) 1:20000
- Exposure time
- 180 seconds
- Compatible with
- all chemiluminescence imagers and X-ray film detection
- Optimized for
- attaining low background and high signal to noise ratio
Applications
- Western Blotting