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        • LightWave™ Plus - ECL HRP, image 1

        LightWave™ Plus - ECL HRP

        Western blotting substrate

        LightWave™ is our product line of ECL HRP substrates for Western blotting.

        Our double enhancer proprietary technology allows for modulation of signal intensity and signal duration. 

        Each LightWave™ substrate is at the top of its respective market segment regarding performance/price ratio.

        Attachments

        Western Blot General Protocol
        Application Note
        General Manual
        Troubleshooting
        Molecular Biology Production Collection

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        Properties

        LightWave is our product line of ECL HRP substrates for Western blotting. Our double enhancer proprietary technology allows for modulation of signal intensity and signal duration.

        LightWave™ Plus Features

        • Mid femtogram detection
        • Extended signal duration
        • High range flexibility
        • Working solution stable for at least three days
        • The perfect ECL formulation combining great sensitivity and long signal duration
        • Signal duration 25 hours

        Characteristics

        Suggested antibody dilutions
        Primary Ab 1:1000 - 1:15,000; Secondary Ab 1:25,000 - 1:150,000
        Signal intensity
        high
        Signal duration
        extended
        Protein abundance
        media
        Compatible with
        all chemiluminescence imagers and X-ray film detection
        Optimized for
        attaining low background and high signal to noise ratio

        Applications

        • Western Blotting

        Product Variants

        Multiple versions of LightWave™ Plus - ECL HRP are available.

        Product Variants

        2 available results

        1. LightWave™ Plus Western Blotting Substrate 10ml
          LW0003
          LightWave™ Plus Western Blotting Substrate 10ml
          LightWave™ Plus Western Blotting Substrate 10ml
        2. LightWave™ Plus Western Blotting Substrate 250ml
          LW0004
          LightWave™ Plus Western Blotting Substrate 250ml
          LightWave™ Plus Western Blotting Substrate 250ml

        Storage/expiry: One year at room temperature (max. 25°C)

        LightWave™ Plus 

        Western blotting detection of HDAC-1 on Hela cells
        Hela cell lysate from 5 to 0,078 μg
        Ab 1° Rabbit anti HDAC1 1:5000
        Ab 2° Goat anti rabbit 1:50000
        Exposure time: 3 minutes
        Imager: LAS4000 (GEHC)

        Click here to download the GVS Lightwave Manual